Hi Muralidharadan, thanks for your comments, I'm aware it will be tricky, I had a go with some lateral buds of Bambusa oldhamii but as I had only started tissue culture at the tail end of Summer, by the time I had some confidence to try it was mid winter and the material was fairly dormant, I cleaned them in 1% bleach with detergent for five minutes and then made clean cuts and trimmed the buds down. There was some initial growth of the bud or so I perceive and there was no infection but as time passed I could see no growth and over the following two months there seem to be tannin like staining of the media. After further reading I am thinking it will need to be in active growth and am hoping to be able to monitor the plants on a daily basis so will try again in February (our mid summer here and usually just ahead of culm shoot time, the new culms sometimes don't shoot till almost Autumn. I'm tending to think that the plants need to energise themselves from the preceding season this far south in the temperate zone. I can get access to the lab and have less work commitments then and will be able to monitor the plants in culture and clean them if they start to show moulding etc ( as I have only been going to the lab on a weekly basis ),prior to taking explants I'm planing on creating a clean environment and providing bottom heat and long daylight hours to induce shooting and active growth and reduce infection, if as you say systemic infection of material is prevalent then it will indeed be challenging, I am thinking of a few other more esoteric attacks to reduce infection so is a bit of a waiting game here. I have just mixed up some media to induce rooting and am looking forward to getting back to the lab and shifting some plants produced in multiplication stage to rooting and then acclimatization to the nursery, possibly in micro containers inside the hothouse . I am finding this work very fascinating and am having as much fun as a pig in excrement. In a world as troubling as ours at the moment I find great comfort in being a part of the bamboo plantation group and of people actively engaging in doing positive things toward making this planet a better place, bless you all.warm regards Peter j.
>>> "E. M. Muralidharan" <emmurali@gmail.com> 20/09/2008 >>>
Hi Peter,
Tissue culture of bamboo from explants taken from adult clumps will be much
more difficult than that of seeds or seedlings. You need to overcome the
hurdles of high rates of contamination and difficulty in rooting.
Yet several tropical clumping species are amenable to tissue culture.
Muralidharan, India
On Mon, Sep 15, 2008 at 7:42 AM, Peter Johnson
<peterj-hrt@nmit.vic.edu.au>wrote:
> Hi Luiz, I have been learning and practicing tissue culture with bamboo
> here in Melbourne Australia with thoughts of producing bamboo for forest
> projects. I am having some good success at early stages starting with seed
> and then going on to multiplication stage and am yet to change media for
> root development and then moving plants to hot house and then outside world.
> I am using tropical clumping bamboo that is available from seed as practice
> and am then wanting to experiment with taking material from
> ex- plant material when it is in active growth later in our Summer (
> February- March). I have found it pretty easy to get plants going from seed
> when the seed is Viable and cleanable but have not had much of a go with
> ex-plants ( the few I tried were not vigorously growing and just sat there
> doing nothing, others I didn't succeed with getting them clean and produced
> fabulous moulds etc) I'd be interested to hear how you go.
> All the best warm regards Peter.
>
> >>> "Luiz A. Gallo" <luagallo@esalq.usp.br <luagallo%40esalq.usp.br>>
> 12/09/2008 >>>
>
> I every one. Im starting a new project for bamboo micropropagations for
> reflorestation of degradated areas. Some one who wants to help me with
> protocols will be welcome and very good to me and my lab.
> Here in Brazil we start to have a serius thouths about bamboos and its use
> as fuel, constructions and biomass accumulation. thanks
>
> [Non-text portions of this message have been removed]
>
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